Gossip meeting “Re-purposing everyday objects for microscopy”
Tuesday 8th June 2021
NOT FINISHED – Lots more images and notes are needed
Paul Smith introduced this meeting, which was held via Zoom and attracted a number of participants from the USA.
Edwin Davies recommends dentist’s root-canal drills as manipulators/prodders that have the right flexibility for moving objects around under low power.
Dentist’s root-canal drills
Edwin uses Pasteur pipettes as holders for the drills.
Pasteur pipette holders for root-canal drills
Mike Gibson talked us through his PowerPoint presentation on Transmitted blue-light fluorescence microscopy using a modified photographic slide projector:
Click the arrows to move through the slides. Click the symbol at bottom right for a larger version.
Graham Matthews showed a home-made adapter constructed to permit repurposing of Watson substage condensers for use with a Leitz Dialux microscope (and other Leitz microscopes using the slide-in dovetail mount). The adapter is a prototype constructed from 40 thou polystyrene sheet.
Watson condenser in Watson mount and Graham’s adapter for the Leitz Dialux microscope
Watson condenser with Watson mounting ring removed
Watson condenser in Dialux condenser adapter
Watson condenser in situ on Dialux microscope
Alan Wood showed items that he has made from a pudding bowl, a packet of cornflakes, a greeting-card wrapper and the window from a packet of chocolate eclairs.
Pudding bowl, cornflakes packet and wrappers
White plastic pudding bowls are not as easy to find as they used to be, but they are still available on Amazon and eBay. Alan uses them as diffusers and reflectors to produce shadowless illumination from an upturned LED ring-light, after removing the base. A cylinder of white paper and a piece of white foamboard with a circular hole help to control stray light.
Construction of shadowless illuminator from pudding bowl and LED ring-light
The illuminator rests on the base of a stereomicroscope or a copying stand.
Shadowless illuminator under a stereomicroscope
Bluebottle (Calliphora vomitoria) using shadowless illumination
Wristwatch mechanism using shadowless illumination
The bottomless bowl can also be used as a spacer to produce dark-ground illumination from an inverted ring-light, with a black background and a piece of black foamboard.
Construction of dark-ground illuminator
Dark-ground illuminator under a stereomicroscope
Housefly wing using dark-ground illumination
Larva of a mosquito (Culex pipiens) using dark-ground illumination
Like several other amateur microscopists, Alan uses makeshift retarders to change the colours of subjects viewed with polarised light. Sources of suitable plastic films include the wrappers on greetings cards and CDs, and the windows on boxes containing cakes or pies. Some films are better than others, so you need to experiment. Alan used to use GePe glassless 35mm slide mounts to hold his retarders, but they are only just big enough and they have to be positioned carefully. Now he uses a compass cutter to make cardboard annuli from boxes of Kellogg’s Corn Flakes.
Compass cutter and cardboard annuli from a cornflake packet
He attaches a circle of plastic film using glue, and the annuli rest on top of a polarising filter and are easy to rotate and observe the best effect.
Makeshift retarder resting on a polarising filter on a microscope base
Colourless potassium chlorate crystals, viewed with crossed polarisers and a retarder
Paper from a wasp nest, viewed with crossed polarisers and a retarder