Workshop on image processing for microscopy

Saturday 11th November 2017

Graham Matthews and Alan Wood led this afternoon workshop in the PA135 Meeting Room in the Natural History Museum in London.

Alan gave the first presentation, showing how he uses Adobe Photoshop Elements to perform basic image processing on photomicrographs.

Graham gave the second presentation, showing how he uses PaintShop Pro for more advanced image processing.


Click the arrows to page though the slides, click the symbol at bottom right for a bigger (and slower) version.

There are videos embedded in the PowerPoint presentation, and you can also watch them separately:


Smart Photo Fix
Click the arrow to start the video (1 minute 34 seconds), click the icon to the left of “vimeo” for a larger version


Clone tool
Click the arrow to start the video (1 minute 40 seconds), click the icon to the left of “vimeo” for a larger version


Salt and Pepper filter
Click the arrow to start the video (1 minute 31 seconds), click the icon to the left of “vimeo” for a larger version


Flood filling the background
Click the arrow to start the video (22 seconds), click the icon to the left of “vimeo” for a larger version


Removing vignetting with image arithmetic (difference)
Click the arrow to start the video (57 seconds), click the icon to the left of “vimeo” for a larger version


Removing vignetting with image arithmetic (addition)
Click the arrow to start the video (56 seconds), click the icon to the left of “vimeo” for a larger version


Adding a scale bar
Click the arrow to start the video (1 minute 48 seconds), click the icon to the left of “vimeo” for a larger version


Here are some examples of the results achieved during and after the workshop.

Stained section

Les Franchi 1 originalImage 1 – original

Les Franchi 1 colour adjustedImage 1 – colour adjusted

Les Franchi 1 levels adjustedImage 1 – levels adjusted, residual colour cast removed


Tissue section

Les Franchi 2 originalImage 2 – original

Les Franchi 2 colour balance and levels adjustedImage 2 – colour balance and levels adjusted


Diatom

Jacky originalImage 3 – original

Jacky image brighterImage 3 – brighter

Jacky image colours adjusted, background cleanedImage 3 – colours adjusted, background cleaned

Jacky image scale and text addedImage 3 – scale and text added


Ciliate

Ciliate image originalImage 4 – original

Ciliate image background de-speckled, image sharpenedImage 4 – background de-speckled, image sharpened


Ladybird dissection

Ladybird originalImage 5 – original

Ladybird lightenedImage 5 – lightened

Ladybird lightened more, background cleanedImage 5 – lightened more, background cleaned


Mushroom spore print

Spore print originalImage 6 – original

Spore print adjustedImage 6 – adjusted


Bee leg

Bee originalImage 7 – original

Bee cropped and adjustedImage 7 – cropped and adjusted

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